Technology

Let us show you that
it is not only better to use enzymes,
but also how to use enzymes better.

Technology

Let us show you that
it is not only better to use enzymes,
but also how to use enzymes better.

Technology

Let us show you that
it is not only better to use enzymes,
but also how to use enzymes better.

Technology

Let us show you that
it is not only better to use enzymes,
but also how to use enzymes better.

Making green chemistry affordable.

The use of biocatalysts can have major advantages: in increased product quality, reduced downstream processing and a much smaller environmental footprint. This is not enough. Biocatalysis must also be cost competitive. Our focus on process optimization and on enzyme recycling makes CLEA Technologies the right partner to also achieve the cost reduction you are looking for. We have proven this with expensive enzymes like ketoreductases in pharma, with more common enzymes like CalB in pharma and oleochemicals and even with low cost enzymes used in first and second generation biofuels.

We have proven this with our proprietary cross-linked enzyme aggregates and with carrier based enzymes. Our novel ferromagnetic CLEAs (mCLEAs) are not only a cost effective means of enzyme immobilization but are also easily and effectively separated, even at very high flow rates, even in the presence of other solids.

Our drive for perfection and innovation will help you make your enzymatic processes work better and more cost effective. Just ask.


Enzyme immobilization technology

The vast majority of biocatalyst applications still involve single use soluble enzymes. And that is a pity. An immobilized enzyme reduces downstream processing and reduces costs significantly. CLEA Technologies has more than a decade of experience in enzyme immobilization and as no other understands the effect it may have on your process.

CLEA

Cross-Linked Enzyme Aggregates

Carrier-free immobilized enzymes and hence a very high specific activity. Due to the covalent bonds CLEAs are non leaching. Applicable in aqueous and solvent systems. Proprietary technology. Low cost.

mCLEA

Ferromagnetic CLEAs 

Small ferromagnetic particles are incorporated in a CLEA using our proprietary technology. The very high magnetic susceptability makes it easy to separate. Ideal when other solids are present or in emulsions, precipitations and fermentations.

BEADs

Carrier based immobilized enzymes

Variety in carriers and bonding. The influence of the nature of the carrier on the activity of the enzyme and the conversion is often underestimated.


Process technology

Selecting the right catalyst for your process is the first step. This can include the design of -for instance- a combi-CLEA. Optimizing the biocatalytic process is the next important step in our service to you. Not only the standard process parameters like pH, temperature, pressure and (co)solvents are of importance but also the treatment of the biocatalyst. Together, this leads to optimal conversions and extended catalyst lifetime.

Combi-CLEA

Two or more enzymes are combined into one biocatalytst. In many cases we find a synergistic effect in a combi-CLEA as local concentrations of intermediate products are very high, which makes a cCLEA ideal for e.g. cascade reactions. It is also possible to combine other catalysts, like Raney nickel with for instance CalB into one (ferromagnetic) immobilized catalyst.

Process optimization

In many cases, the re-use of immobilized enzymes is key to make biocatalysis commercially viable. Getting the most out of the enzyme is not straightforward. With our experience, immobilization and process optimization will make the difference. For instance in the use of immobilized CalB, we are able to extend the lifetime of the catalyst significantly. If this rings a bell, please give us a call.

Magnetic separation

Until now, using immobilized enzymes in reactions with other solids present has been close to impossible. CLEA Technologies has developed a new method to manufacture ferromagnetic cross-linked enzyme aggregates with a very high magnetic susceptibility, on large scale and at low cost.