Products

Cross-Linked Enzyme Aggregates

Products

Ferromagnetic
Cross-Linked Enzyme Aggregates

Products

Carrier based immobilized enzymes

Products

Soluble enzymes

Products

 Discovery platforms

Products

Cross-Linked Enzyme Aggregates

Products

Carrier based immobilized enzymes

Products

Ferromagnetic CLEAs

Products

Discovery platforms

CLEA Technologies as (immobilized) enzyme supplier

We are not an enzyme manufacturer. For all our products we either use your enzyme or commercially available enzymes. The vast majority of the products we deliver are in the first category: immobilized versions of customer specific enzymes. For example ketoreductases in pharma, amylases and cellulases for bio-ethanol or saccharide isomerases for food. Very diverse enzymes for very diverse applications.

We supply enzymes on gram scale, on kilo scale and on full industrial scale. Usually, these products are customer specific and not on our public portfolio. Some broadly applicable enzymes like CalB and Alcalase (TM), we supply off the shelf as (m)CLEA or on beads.

An overview of all available product are at the bottom of this page.

CLEAs

Cross-Linked Enzyme Aggregates. Carrier-free immobilized enzymes and hence a very high specific activity. Due to the covalent bonds CLEAs are non leaching. Applicable in aqueous and solvent systems. Proprietary technology. Low cost. Apart from the enzymes mentioned in the product overview, we do customer specific immobilizations. Fast and at a very reasonable price. Read more about CLEAs.

mCLEAs

Small ferromagnetic particles are incorporated in a CLEA using our proprietary technology. The very high magnetic susceptability makes it easy to separate. Ideal when other solids are present or in emulsions, precipitations and fermentations.
Read more about mCLEAs.

BEADs

Carrier-bound immobilized enzymes. Variety in carriers and bonding. The influence of the nature of the carrier on the activity of the enzyme and the conversion is often underestimated. Apart from the enzymes mentioned in the product overview, we do customer specific immobilizations. Fast and at very reasonable costs.
Read more about BEADs.

Soluble enzymes

Available as liquid or as lyophilized powders, we provide a wide range of proteases, lipases, ketoreductases, transaminases, nitrile hydratases and nitrilases. Both on small scale for research purposes and on large scale commercial applications. The majority is available both as single enzyme as well as in a discovery platform.

Discovery platforms

Both for research labs and universities, we have developed screening kits of the most popular enzyme families: lipases, proteases, ketoreductases and nitrile hydratases. Recently, we added a special CaLB kit to our portfolio with different forms of immobilization.

Due to our extensive experience in biocatalysis, if the budget is available, we strongly advise to consider our enzyme screening service as in many cases we see projects fail due to a lack of understanding the specific needs of enzymatic reactions.

 

Available products

For quotation requests or orders, please fill out our enquiry form.

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For quotation requests or orders, please fill out our enquiry form.

Source of  Lipase Additional Description Formulation Activity Spec. Catalogue 
Candida antarctica Isoform A Active on hindered alcohols, preference on short and medium chain solution        6,000 TBU/mL FE101
suspension      20,000 TBU/g dw CLEA101
Candida antarctica Isoform B Active on a broad range of substrates solution        5,000 TBU/mL FE102
powder      10,000 TBU/g dw CLEA102ST
powder      30,000 PLU/g dw CLEA102OM
powder        9,000 PLU/g dw CLEA102B4
Candida rugosa Non specific on triglycerids lyophilized      70,000 TBU/g FE103
suspension        1,000 TBU/g dw CLEA103
Thermomyces lanuginosus Non specific on triglycerids solution      60,000 TBU/mL FE104
powder    400,000 TBU/g dw CLEA104
Rhizomucor miehei 1,3-specific, preference for short chain solution        8,000 TBU/mL FE105
Penicillium expansum Active in high alkaline region lyophilized        5,000 TBU/g FE106
Penicillium camembertii Active on mono- and diglycerides lyophilized      50,000 U/g FE107
Penicillium roqueforti 1,3-specific, preference for short and medium chain lyophilized        2,000 TBU/g FE108
Aspergillus niger 1,3-specific lyophilized      10,000 U/g FE109
Burkholderia cepacia Methanol tolerance lyophilized      18,000 TBU/g FE110
Pseudomonas fluorescens Non specific on triglycerids lyophilized      15,000 TBU/g FE111
Rhizopus niveus 1,3-specific, preference for medium and long chain lyophilized      30,000 U/g FE112
Rhizopus oryzae 1,3-specific, broad pH stability lyophilized      10,000 TBU/g FE113
Mucor javanicus Preference for medium  chain length lyophilized        5,000 TBU/g FE114
Alcaligenes sp. 1,3-specific lyophilized      20,000 TBU/g FE115
suspension           300 TBU/mL CLEA115ST
suspension           300 TBU/mL CLEA115OM
Porcine pancreas Pancreatic triacylglycerol lipase lyophilized        4,000 TBU/g FE116
Pseudomonas stutzeri 1,3-specific lyophilized      70,000 TBU/g FE117
suspension           500 TBU/mL CLEA117ST
suspension           500 TBU/mL CLEA117OM
Porcine pancreas phospholipase A2 Hydrolyzes the β-ester bond of glycerophospholipids solution      10,000 U/mL FE150
Thermomyces lanuginosus phospholipase A1 Broader specificity than phospholipase A2 lyophilized      10,000 U/g FE151
Source of Protease Additional Description Formulation Activity Spec. Catalogue 
Bacillus licheniformis, Alcalase* Subtilisin serine endo-protease solution            2.5 AU/mL FE201
       100 NAGEU/mL
*Alcalase is a registered trademark of Novozymes A/S suspension       400 NAGEU/g dw CLEA201ST
Bacillus clausii Serine protease, active under high alkaline pH solution            2.5 AU/mL FE202
      150 NAGEU/mL
powder       400 NAGEU/g dw CLEA202ST
Bacillus lentus Serine protease, active under high alkaline pH and elevated temperatures solution            2.5 AU/mL FE203
      125 NAGEU/mL
powder       300 NAGEU/g dw CLEA203ST
Carica papaya (Papain) Cysteine protease solution       850 HbU/mL FE204
      500 NBAEU/mL
CLEA with improved thermal stability suspension       100 NAGEU/g dw CLEA204ST
Bacillus amyloliquefaciens CLEA is specifically effective under high alkaline and high temperature conditions lyophilized          75 NAGEU/g FE205
powder          50 NAGEU/g dw CLEA205ST
Bacillus stearothermophilis Metallo protease – “Thermolysine”, highly thermostable and most active in neutral pH lyophilized          30 NAGEU/g FE206
Aspergillus oryzae – Acidic Acid peptidase, specifically suitable for secondary hydrolysis of peptides. lyophilized          70 CU/g FE207
Aspergillus oryzae – Alkaline Alkaline peptidase, specifically suitable for secondary hydrolysis of peptides lyophilized       200 CU/g FE208
Aspergillus sp. – Peptidase Endo protease, high amino- and carboxy-peptidase activity lyophilized       120 CU/g FE209
Bacillus licheniformis Subtilisin type serine endo-protease solution       150 NAGEU/mL FE213
powder       300 NAGEU/g dw CLEA213ST

KRED Screening Study

We offer a screening study. We have 200 KREDs in house and a rapid screening method. All we need is the target alcohol reference. We do not need to know what the alcohol is or what your target reaction is, we just ask for a sample solution with a given concentration against an MTA. In return we will tell you whether we have a candidate enzyme that can do the conversion for you.

Most KRED screening kits on the market are developed based on protein engineering, in one way or the other. We provide native enzymes that are evenly sampled from the nature. In other words, all the enzymes in the kit are unique and the members share a maximum of 32% identity. This diversity increases the chance to find a hit for your unique substrate.

Another key feature of our approach is that we provide the screening study free of charge. This means that you do not run any loses if the screening result is negative.

The whole study, from the initial screening to process optimization can be a long and costly journey. But we like to keep things simple and transparent. We give full commitment to your project and expect you to do the same. With intense communication and clear demands, the study can be finalized within 3-4 months. The costs depend largely on the amount of man hours that has to be invested in the project, and therefore will vary from one project to the other. But we can assure you that it will not cost millions of euros.

For more information, please download our brochure or drop us a note.

 

Source of NHase Formulation Activity Spec. Catalogue
Rhodopseudomonas palustris suspension 400-600  U/mL CLEA601
Rhodopseudomonas palustris suspension 400-700  U/mL CLEA602
Rhizobium meliloti suspension 500-600  U/mL CLEA603
Roseobacter denitrificans suspension 300-400  U/mL CLEA604
Bordetella petrii suspension 300-400  U/mL CLEA605
Stappia aggregata suspension 400-500  U/mL CLEA606
Silicibacter lacuscaerulensis suspension 500-600  U/mL CLEA607
Phaeobacter gallaecienis suspension 400-500  U/mL CLEA608
Roseobacter litoralis suspension 500-1000  U/mL CLEA609
Nitriliruptor alkaliphilus suspension 1000-3000  U/mL CLEA610
Enzyme Remarks
Catalase available from catalogue as soluble enzyme and as CLEA (product codes FE807 and CLEA807)
Hydroxynitrile lyase available upon request
Lactase available upon request
Nitrilase available upon request
Oxynitrilase available from catalogue as soluble enzyme and as CLEA (product codes FE502 and CLEA502)
Penicillin G amidase available from catalogue as soluble enzyme and as CLEA (product codes FE301 and CLEA301)
Transaminase available upon request
Enzyme Type Content Remarks Catalogue
Lipase Soluble Enzymes A set of 19 most used lipases. 1 g or 10 ml each Contains a wide variety of lipases suitable for esterification, interesterification, acidolysis, alcoholysis and aminolysis reactions FE1DP1
Immobilized Enzymes A set of 9 CLEAs, 6 of which are the most common comercially available lipases for organic synthesys: CalA, CalB, Candida Rugosa, TLL, Alcaligenes Lipase, Pseudomonas Stuzeri. 1 g or 10 ml of each product Excellent starting point for lipase screening where immobilized enzymes are a neccesity CLEA1DP1
CalB kit 2 CLEAs (one for aequous and one for organic media), 1 mCLEA and several bead immobilized versions Excellent starting point for esterification and amidation research CLEA102DP
Protease Soluble Enzymes A set of 10 comercially available proteases, 1 g or 10 ml each Consists of a highly relevant selection of enzymes available from R&D amounts to industrial scale FE2DP1
Immobilized Enzymes A set of 6 CLEAs, 1 g each. All from commercially available proteases Specifically constructed as rapid screening tool to identify the best immobilized protease for your application CLEA2DP1
Nitrile hydratase Immobilized Enzymes A set of 10 NHase CLEAs Excellent stability, best alternative to whole cell catalyst CLEA6DP1