mCLEAs

Ferromagnetic Cross-Linked Enzyme Aggregates

mCLEAs

Ferromagnetic Cross-Linked Enzyme Aggregates

Ferromagnetic Cross-Linked Enzyme Aggregates

mCLEAs are a special form of Cross-Linked Enzyme Aggregates (read more) in which, using our proprietary knowledge, small ferromagnetic particles are incorporated in the matrix. Until now, using immobilized enzymes in reactions with other solids present has been close to impossible. The main characteristics of a CLEA remain: carrier free, high specific activity, tunable, low cost.

Until now, there were two reasons why this technology was not applied on industrial scale: cost of the ferromagnetic particles and the low magnetic susceptibility. CLEA Technologies has developed a new method to manufacture ferromagnetic cross-linked enzyme aggregates with a very high magnetic susceptibility, on a large scale and at low cost. Patented technology.

We see a very broad application field for our mCLEAs: replacing ordinary CLEAs in various processes as the separation is so easy, in first and second generation biofuels, either in SHF or SSF; in various food applications or in pharma where reaction products precipitate, as for instance in cephalosporins.

Designing an mCLEA of your enzyme is still a few weeks work. This will be enough for you to evaluate the potential application. In a second stage, we will optimize the mCLEA in your reaction. Bringing the cost down and/or increasing the activity and stability. Scaling up can be done at CLEA Technologies, under a license agreement at your place or outsourced to a toll manufacturer.

We are open to work with enzyme users and enzyme manufacturers. Please contact us.


Magnetic Separation

Until now, using immobilized enzymes in reactions with other solids present has been close to impossible. CLEA Technologies has developed a new and proprietary method to manufacture ferromagnetic cross-linked enzyme aggregates with a very high magnetic susceptibility, on large scale and at low cost.

Ferromagnetic enzymes, either as CLEA or on single-enzyme scale are known from literature but have not found any significant application outside the scientific world. Our mCLEAs, combining a high ferromagnetic strength with a low costs make it applicable on industrial scale.

The use of ferromagnetic catalysts and magnetic separation as such, like for instance Raney nickel, is known in industry. Using rare earth permanent magnets in a kind of flow cell as, for instance,in the Eclipse Magnets Automag series, gives a very high recovery rate, even at flow rates that are completely acceptable to the industry.